EFFECTS OF BACTERIOCIN AND ORGANIC ACID ON Listeria monocytogenes IN FEED

Listeria monocytogenes is a Gram-positive, psychrotropic, facultative anaerobic pathogen and it is the etiological agent of listeriosis, a severe foodborne disease of major public health concern. Listeria monocytogenes has high ability to adapt adverse condition. Therefore, the control of Listeria monocytogenes in foods and feeds is very difficult. N this purpose, organic acids and bacteriocins have been used for many years in preservation of foods against pathogen contamination. In this considering, it was aimed to determine the effects of bacteriocin, organic acid, and mixtures of them on the Listeria monocytogenes in this study. Feeds were divided into six experimental groups that consist of control, 150 mg/kg bacteriocin (B150), 300 mg/kg bacteriocin (B300), 3g/kg organic acid (OA), 150 mg/kg bacteriocin + 3 g/kg organic acid (B150+OA) and 300 mg/kg bacteriocin + 3 g/kg organic acid (B300+OA). Listeria monocytogenes cultures were added by spraying on feed samples in sterile bags and were counted by inoculating onto agars at 0, 7, 15, 21 and 28 days. At the end of this study, bacteriocin and organic acid supplementations have been found to have positive effects on Listeria monocytogenes contamination. But the best antilisterial effects was achieved by combined use of bacteriocin and organic acid.